Tobias Baumgart and Shu Yang
The deformation of cell membranes couples with spatial distributions of membrane proteins and lipids. Current model membrane approaches studying curvature sensing are limited to positive curvatures and often involve complex setups. To overcome these challenges, solid- supported wavy membrane were fabricated by a combination of soft lithography and wet etching. We demonstrated that endocytotic proteins and the protein cholera toxin show curvature sorting on this engineered wavy membranes, whereas a control protein (streptavidin) showed no sorting.
Surface topography and curvatures measured via AFM (left), Lipids and peripheral proteins on wavy membranes imaged via epi-fluorescence microscopy (right)
Langmuir, August 2012